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MICROSCOPY

http://biology.clc.uc.edu/fankhauser/Labs/Microscope/Microscope_Features&Care.htm

I). Parts of the Microscope

    A). Backbone of the microscope are:

            i). base

            ii). arm

            iii). stage

                        a). slides are place onto the stage

                        b). stage clips hold the slides.

                        c). mechanical stage knobs move the stage

    B). Substage Light Source

            i). Fixed light source with dimmer

            ii). condenser

              iii). iris diaphragm lever

    C). Focus

            i) course focus knob

            ii). fine focus knob

    D). Magnifying Power

            i). ocular lens (10x)

            ii). objective lens

III). Magnification

       A).   Types of lens

i). Scanning Lens:

ii). High Power Lens:

iii). Oil-Immersion Lens:

A). Working distance  

B). Field of view:  

C). Resolution:  

D). Parfocal capability:  

If you cannot see or focus your specimen try this:

 1). Look at you objective lens.

If it is more than a centimeter from your slide, your working distance is too great.

2). If you were focused on your specimen and you lost it when you changed power.

Your specimen is no longer in your field of view move the slide slightly you will find it.

3). You just see pink.

You have not focused your slide until you can see individual cells.

4). You were focused but you are no longer focused at higher powers.

The parfocal ability is low; just adjust the focus.

5). You see a dark half moon shape.

You have not set the lens. Make sure the lens clicks.

6). You cannot find the specimen.

Make sure the specimen is under the lens and not off to the side.

7). The slide is too light or too dark.

Adjust the dimmer switch or the diaphragm lever to change contrast. Try a slide that was stained darker.